05-1530-M

Anti-Importin beta-1 Antibody, clone 3H14

clone 3H14, from mouse

• Synonyme(s) : Karyopherin subunit beta-1, Nuclear factor P97, importin 1, importin 90, importin beta-1 subunit, karyopherin (importin) beta 1, karyopherin beta 1
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 3H14, monoclonal
• Espèces réactives: human
• Technique(s): immunocytochemistry: suitable; western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_002256.2
• Numéro d'accès UniProt: Q14974
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... KPNB1(3837)

Description

Description générale

Importin beta-1 (also known as Importin subunit beta-1) facilitates nuclear protein import. This occurs either though association with a nuclear localization signal (NLS) and an adapter protein , such as an importin-alpha subunit, which which binds to nuclear, or directly where the protein acts as autonomous nuclear transport receptor.
Import of macromolecules containing a NLS requires a heterodimer of importin alpha and beta subunits also called as karyopherins. Importin alpha binds the cytoplasmic NLS-containing protein and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. Movement through the nuclear pore is mediated by the small GTP binding protein Ran via an energy requiring mechanism. Using nucleotide triphosphates and Ran, the nuclear transport complex moves into the nuclear pore and the importin subunits dissociate. Importin alpha and its passenger protein enter the nucleoplasm while importin beta remains at the pore.

Spécificité

This antibody recognizes Importin beta-1.

Immunogène

Epitope: Unknown

Recombinant protein corresponding to human Importin beta-1.

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Metabolism & Binding Proteins

Use Anti-Importin beta-1 Antibody, clone 3H14 (Mouse Monoclonal Antibody) validated in WB, ICC to detect Importin beta-1 also known as Karyopherin subunit beta-1, Nuclear factor P97.

Qualité

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected Importin beta-1 on 10 µg of HeLa cell lysate.

Description de la cible

~ 97 kDa

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
HeLa cell lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable