04-1571-I
Anti-RNA polymerase II subunit B1 (phospho CTD Ser-2), clone 3E10 (rat monoclonal).
culture supernatant, clone 3E10, from rat
Synonyme(s) :
DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rat
Niveau de qualité
Forme d'anticorps
culture supernatant
Type de produit anticorps
primary antibodies
Clone
3E10, monoclonal
Espèces réactives
mouse
Réactivité de l'espèce (prédite par homologie)
human (immunogen homology)
Technique(s)
ChIP: suitable
ELISA: suitable
western blot: suitable
Isotype
IgG1κ
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
phosphorylation (pSer2)
Informations sur le gène
human ... POLR2B(5431)
Description générale
RPB1 (RNA polymerase II subunit B1) is the catalytic component of RNA polymerase II which synthesizes mRNA and non-coding RNAs. During transcription, elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of RPB1, which acts as an assembly platform for factors regulating transcription initiation, elongation, termination and mRNA processing. Phosphorylation occurs mainly at residues ′Ser-2′ and ′Ser-5′ of the tandem 7 residue repeats in the C-terminal domain (CTD), which activates Pol II. This phosphorylation also can occur at ′Ser-7′ of the heptapepdtide repeat. This antibody recognizes the ′Ser-2′ CTD residue of RPB1.
Spécificité
Demonstrated to react with human RBP1 in Ni et al., 2011. Transcription 2:5, 237-242
This antibody recognizes RNA Polymerase II subunit B1 phosphorylated at Ser2 of the C-terminal domain (CTD).
Immunogène
Epitope: Phosphorylated Ser2 at the C-terminal domain (CTD)
Ovalbumin-conjugated linear peptide corresponding to human RNA Polymerase II subunit B1 phosphorylated at Ser2 of the C-terminal domain (CTD).
Application
Anti-RNA polymerase II subunit B1 (phospho CTD Ser-2), clone 3E1 (rat monoclonal) is a highly specific rat monoclonal antibody & that targets RNA Polymerase & has been tested in western blotting.
Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to immunoprecipitate RNA polymerase II subunit B1 (phospho-CTD Ser-5) in ChIP (Chapman, R., et al. (2007). Science. 318(5857):1780 -1782.).
Demonstrated to react with human RBP1 in Ni et al., 2011. Transcription 2:5, 237-242
Demonstrated to react with human RBP1 in Ni et al., 2011. Transcription 2:5, 237-242
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Qualité
Evaluated by Western Blotting in untreated and lambda phosphatase treated NIH/3T3 cell lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected RNA Polymerase II subunit B1 (phospho CTD Ser-2) in untreated NIH/3T3 cell lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected RNA Polymerase II subunit B1 (phospho CTD Ser-2) in untreated NIH/3T3 cell lysate.
Description de la cible
~220 kDa observed
Liaison
Replaces: 04-1571
Forme physique
Unpurified
Unpurified rat monoclonal IgG1κ tissue culture supenatant with 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Remarque sur l'analyse
Control
Untreated and lambda phosphatase treated NIH/3T3 cell lysate
Untreated and lambda phosphatase treated NIH/3T3 cell lysate
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
In vivo live imaging of RNA polymerase II transcription factories in primary cells.
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Genes & Development, 27, 767-777 (2013)
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Nucleic acids research, 47(9), 4462-4475 (2019-03-14)
The general transcription factor P-TEFb, a master regulator of RNA polymerase (Pol) II elongation, phosphorylates the C-terminal domain (CTD) of Pol II and negative elongation factors to release Pol II from promoter-proximal pausing. We show here that P-TEFb surprisingly inhibits
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