03-903

GFP, FLAG-tagged SNRNP70 mRNA

• Code UNSPSC : 41116158
• Nomenclature NACRES : NA.52

Propriétés

• Conditionnement: 1 ea of 50 μg
• Niveau de qualité: 100
• Fabricant/nom de marque: Millipore
• Mode de croissance: N/A
• Technique(s): RIP: suitable; cell culture | mammalian: suitable; flow cytometry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Conditions d'expédition: dry ice
• Température de stockage: -65 to -96°C

Description

Application

Both mRNAs and non-coding RNAs interact with RNA-binding proteins and regulate critical cellular processes and their dysregulation contributes to multiple diseases. Thus, analyzing RNA-protein interactions are critical for understanding the mechanism of diseases and designing clinical strategies.
RNA-binding protein immunoprecipitation (RIP) has been used to interrogate RNA binding proteins (RBP) associated with specific RNA molecules. However, the experiments are not feasible because of the unavailability of antibodies for the particular RBP or low expression of the RBP. To overcome these issues epitope tags have been used for RIP experiments.

Caractéristiques et avantages

The GFP, FLAG-tagged SNRNP70 mRNA was developed to enable to perform control RIP experiments with RIPAb+ FLAG (Cat. No. 03-901) or RIPAb+ GFP (Cat. No. 03-902) RIP validated antibody and primer kits, and Magna RIP kit (Cat. # 17-700).

Stockage et stabilité

Store at -80ºC

Autres remarques

The high amount of GFP-FLAG-tagged SNRNP70 protein is expressed immediately after transfection to cells and the well-known specific interaction of SNRNP70 and U1snRNA can be used for control RIP experiments with anti-GFP or anti-FLAG antibodies.

Clause de non-responsabilité

Sauf indication contraire dans notre catalogue ou toute autre documentation associée au(x) produit(s), nos produits sont uniquement destinés à la recherche et ne sauraient être utilisés à d'autres fins, ce qui inclut, sans s'y limiter, les utilisations commerciales non autorisées, les utilisations diagnostiques in vitro, les utilisations thérapeutiques ex vivo ou in vivo, ou tout type de consommation ou d'application chez l'être humain ou chez l'animal.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable