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03-0176-00

Millipore

SMC® Human IL-10 High Sensitivity Immunoassay Kit

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Description

LLOQ range: 0.1-0.2 pg/mL, refer to CoA forlot-specific LLOQ

Espèces réactives

rat, mouse, feline, canine, nonhuman primates

Fabricant/nom de marque

SMC®

Technique(s)

single molecule counting: suitable

assay range

inter-assay cv: <20%
intra-assay cv: <15%
(Intra-assay n=20; Inter-assay n=5)

Température de stockage

2-8°C

Description générale

The SMC® Human IFN-b1 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format.
Assay protocols are similar to existing sandwich ELISA methods with two key differences:
1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification.
2) The Erenna and SMCxPRO® immunoassay systems detect analytes using Single Molecule Counting (SMC®) technology.

Spécificité

Species Reactivity
Although IL-10 was detectable in non-human primate, mouse, rat, canine, and feline plasma, the assay has not been validated for these species.

Accuracy:
  • Minimum of 5 serum samples spiked with average recovery of 87% with a range of 84-93%
  • Minimum of 5 plasma samples spiked with average recovery of 90% with a range of 82-97%

Application

The sensitivity of the Single Molecule Counting (SMC®) technology gives the SMC® Human IL-10 High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IL-10 in human serum and plasma.

Conditionnement

96 well assay

Composants

  • Assay Buffer
  • IL-10 Coated Beads
  • Standard Diluent
  • IL-10 Detection Antibody
  • IL-10 Standard
  • 10X Wash Buffer
  • Buffer D
  • Elution Buffer B

Informations légales

SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictogrammes

Skull and crossbonesHealth hazard
GHS06,GHS08

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Certificats d'analyse (COA)

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Contenu apparenté

Power of High Sensitivity Assays in Immunology Research | SMC® Technology

See how you can enhance your immunology research through the power of high sensitivity immunoassays by harnessing Single Molecule Counting (SMC®) technology to precisely characterize interferons, interleukins, and immunoglobins.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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