235474

Acridine Orange base

Dye content 75 %

• Synonyme(s) : 3,6-Bis(dimethylamino)acridine, Solvent Orange 15
• Formule empirique (notation de Hill) : C₁₇H₁₉N₃
• Numéro CAS: 494-38-2
• Poids moléculaire : 265.35
• Numéro C.I. (Colour Index): 46005
• Numéro MDL: MFCD00005029
• Code UNSPSC : 12171500
• ID de substance PubChem : 24854106
• Nomenclature NACRES : NA.47

Propriétés

• Forme: powder
• Composition: Dye content, 75%
• Technique(s): microbe id | staining: suitable
• Pf: 165 °C (dec.) (lit.)
• Solubilité: 1 M HCl: 1 mg/mL, clear
• λmax: 488 nm
• ε (coefficient d'extinction): ≥14000 at 227-233 nm; ≥20000 at 289-295 nm; ≥35000 at 486-492 nm; ≥40000 at 266-272 nm
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: room temp
• Chaîne SMILES: CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C
• InChI: 1S/C17H19N3/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14/h5-11H,1-4H3
• Clé InChI: DPKHZNPWBDQZCN-UHFFFAOYSA-N

Description

Description générale

Acridine Orange base (3,6-Bis(dimethylamino)acridine) is a cell-permeable fluorescent dye that binds to nucleic acids. It emits green fluorescence when bound to dsDNA and red fluorescence when bound to ssDNA or RNA. It is also known to be a positive solvatochromic dye.

Application

Acridine orange has also been used as a lysosomal dye. It is widely used to detect the presence of homeopathic potencies.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)