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U0880

Sigma-Aldrich

Uricase from Candida sp.

recombinant, expressed in E. coli, lyophilized powder, ≥2 units/mg solid

Synonym(s):

Urate: oxygen oxoreductase

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About This Item

CAS Number:
9002-12-4
Enzyme Commission number:
1.7.3.3 (BRENDA, IUBMB)
MDL number:
MFCD00082126
UNSPSC Code:
12352204
PubChem Substance ID:
24900619
NACRES:
NA.54

recombinant

expressed in E. coli

form

lyophilized powder

specific activity

≥2 units/mg solid

mol wt

35 kDa by SDS-PAGE

storage temp.

−20°C

InChI

1S/C18H23N4O14P.H3N/c23-5-7-14(13(28)16(34-7)22-4-2-10(25)20-18(22)30)36-37(31,32)33-6-8-11(26)12(27)15(35-8)21-3-1-9(24)19-17(21)29;/h1-4,7-8,11-16,23,26-28H,5-6H2,(H,31,32)(H,19,24,29)(H,20,25,30);1H3

InChI key

VPXDHZMLJOJKOX-UHFFFAOYSA-N

General description

Uricase deficient mice develop uric acid nephropathy and diabetes insipidus which can be mitigated with recombinant uricase treatment.
Uricase is a homo-tetrameric protein with its active sites at the head-to-tail monomer-dimer interface.

Application

Uricase from Candida sp. has been used:
  • to eliminate urate in plasma samples and evaluate the contribution of urate to the total plasma antioxidant capacity
  • to intraperitoneally inject the mice to study the role of uric acid in fibrin extracellular traps (ET) formation
  • for elimination of uric acid in ferric reducing/antioxidative power (FRAP) assay to measure the total antioxidative capacity of human plasma sample; in uricase-based biosensors

Biochem/physiol Actions

Uricase participates in purine catabolism. It catalyzes the conversion of highly insoluble uric acid into 5-hydroxyisourate. Accumulation of uric acid causes liver/kidney damage or chronically causes gout. In mice, a mutation in the gene encoding uricase causes a sudden increase in uric acid. Mice, deficient in this gene, exhibit hyperuricemia, hyperuricosuria, and uric acid crystalline obstructive nephropathy.

Unit Definition

One unit will oxidize 1.0 μmole of uric acid to allantoin per minute at pH 8.5 at 25 °C.

Physical form

Powder; contains citrate and sucrose

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Journal of the American Society of Nephrology : JASN, 12(5), 1001-1009 (2001-04-24)
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Pediatrics, 131(1), e309-e312 (2012-12-05)
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