GE17-0403-03
HiTrap® Protein A High Performance
Cytiva 17-0403-03, pack of 5 × 5 mL
Synonym(s):
HiTrap Protein A HP, high performance column, antibody purification
About This Item
Recommended Products
ligand
native protein A
packaging
pack of 5 × 5 mL
manufacturer/tradename
Cytiva 17-0403-03
storage condition
(20% Ethanol)
parameter
42 psi
bed size
16 mm × 25 mm
bed volume
5 mL
column I.D.
16 mm
size
avg. part. size
34 μm
cleaning
2-10
working range
3-9
storage temp.
2-8°C
General description
HiTrap® Protein A HP is a prepacked ready to use, column for preparative purification of monoclonal and polyclonal antibodies. The special design of the column, together with the medium provide fast, simple and easy separations in a convenient formulation.
Features and Benefits
- High binding capacitiy of 20 mg IgG/mL medium and fast kinetics.
- 34 μm bead size for high-performance, high-resolution purifications.
- Designed for use with syringe, pump, or chromatography system such as AKTA design or FPLC™ System.
- Stable over pH 3 to 9 for use, and 2 to 10 for cleaning.
- Robust, easy-to-use method.
Storage and Stability
Analysis Note
Other Notes
Legal Information
related product
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Articles
This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from GE Healthcare.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
Protocols
Protein A is derived from a strain of Staphylococcus aureus and contains five regions that bind to the Fc region of IgG. As an affinity ligand, protein A is coupled to Sepharose so that these regions are free to bind IgG.
This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service