GE29-0485-76
HiTrap® Protein A High Performance
Cytiva 29-0485-76, pack of 1 mL
About This Item
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packaging
pack of 1 mL
manufacturer/tradename
Cytiva 29-0485-76
availability
not available in North America
storage temp.
2-8°C
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General description
Application
Features and Benefits
- High binding capacitiy of 20 mg IgG/mL medium and fast kinetics.
- 34 μm bead size for high-performance, high-resolution purifications.
- Designed for use with syringe, pump, or chromatography system such as ÄKTA design or FPLC System.
- Stable over pH 3 to 9 for use, and 2 to 10 for cleaning.
- Robust, easy-to-use method.
Storage and Stability
Legal Information
related product
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
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Articles
This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from GE Healthcare.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
Protocols
This page shows general instructions for affinity purification using HiTrap columns from GE Healthcare.
Protein A is derived from a strain of Staphylococcus aureus and contains five regions that bind to the Fc region of IgG. As an affinity ligand, protein A is coupled to Sepharose so that these regions are free to bind IgG.
This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
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