Skip to Content
Merck
All Photos(1)

Documents

G005880

GC Analysis of Polynuclear Aromatic Hydrocarbons (PAHs) in Grilled Hamburger on SPB®-608 (20 m x 0.18 mm I.D., 0.18 μm) after QuEChERS Cleanup using Supel™ QuE Z-Sep, Fast GC Analysis

suitable for GC, application for SPE

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

technique(s)

gas chromatography (GC): suitable
solid phase extraction (SPE): suitable

test parameters

sample preparation: Dispersive (QuEChERS)
sample/matrix: grilled hamburger spiked with PAHs at 100 ng/g
extraction tube: Sodium chloride/magnesium sulfate (55294-U)
extraction process: add 25 mL acetonitrile, contents of unbuffered salt tube (55294-U); shake for 5 minutes; centrifuge for 5 minutes at 3200 rpm
clean-up tube: Supel QuE Z-Sep tube (55403-U)
clean-up process: transfer 3 mL of acetonitrile layer into a Supel QuE Z-Sep cleanup tube (55403-U); shake 1 minute; centrifuge for 3 minutes at 3400 rpm; remove 1 mL supernatant for GC-MS analysis
column: SPB-608, 20 m x 0.18 mm I.D. x 0.18 μm
oven: 60 °C (1 min), 25 °C/min to 275 °C, 10 °C/min to 300 °C (13 min)
inj. temp.: 265 °C
detector: MS-SIM
MSD interface: 300 °C
carrier gas: helium, 1.5 mL/min constant flow
injection: 1 μL splitless (1 min.)
liner: 4 mm I.D. FocusLiner™ with taper
sample: grilled hamburger extract

suitability

application for SPE

application(s)

food and beverages

Analysis Note

Sample pretreatment: Place 5 g ground hamburger sample into 50 mL centrifuge tube (55248-U). Spike with 100 ng/g target PAHs and internal standards. Let sit 10 min. Add 5 mL water and homogenize.
This application demonstrates the use of dispersive SPE or QuEChERS for the extraction and cleanup of grilled hamburger for GC-MS analysis of polynuclear aromatic hydrocarbons (PAHs). Z-Sep sorbent was used in the cleanup step and found to reduce background and yield recoveries >80% for a majority of the PAHs tested.

Other Notes

Study Schedule

Legal Information

FocusLiner is a trademark of SGE Analytical Science Pty Ltd.
SPB is a registered trademark of Merck KGaA, Darmstadt, Germany
Supel is a trademark of Sigma-Aldrich Co. LLC

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service