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G004912

GC Analysis of Volatiles in Multifloral Honey on SUPELCOWAX 10 after SPME using a 50/30 μm DVB/Carboxen/PDMS Fiber

suitable for GC, application for SPME

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technique(s)

gas chromatography (GC): suitable
solid phase microextraction (SPME): suitable

test parameters

sample/matrix: 5 g multifloral honey + 5 mL bidistilled water in a 20 mL vial, allowed to equilibrate at 50 °C for 15 min with magnetic stirring
SPME fiber: 2 cm 50/30 μm divinylbenzene/carboxen on polydimethylsiloxane (57348-U)
extraction: headspace, 50 °C for 40 min with magnetic stirring
desorption process: 250 °C for 2 min
column: SUPELCOWAX 10, 30 m x 0.25 mm I.D., 0.25 μm (24079)
oven: 35 °C (8 min), 6 °C/min to 60 °C, 4 °C/min to 160 °C, 20 °C/min to 200 °C (1 min)
inj. temp.: 250 °C
MSD interface: 230 °C
scan range: 35-300 m/z
carrier gas: helium, 1 mL/min
liner: 0.75 mm I.D., SPME
sample: (Honey)

suitability

application for SPME

application(s)

agriculture
food and beverages

Analysis Note

In this application, headspace SPME (HS-SPME) using a Carboxen/PDMS/DVB fiber coupled with gas chromatography-mass spectrometry (GC/MS) was used for the characterization of the volatile fraction of honey samples. Using SPME it was possible to obtain and distinguish the chemical fingerprints of different types of honey. The relatively polar SUPELCOWAX 10 GC column provides excellent separation of these polar compounds.

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Description

    Nonanal
    Furfural
    Decanal
    Benzaldehyde
    Phenylacetaldehyde
    Phenylethyl Alcohol
    Nonanoic acid

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