G001730
HPLC Analysis of Peptides on Discovery® BIO Wide Pore C18, 0-16 Min. Window
application for HPLC
About This Item
technique(s)
HPLC: suitable
test parameters
column: Discovery BIO Wide Pore C18, 15 cm × 4.6 mm I.D., 5 μm particles (568222-U)
mobile phase: [A] (95:5), 0.1% TFA in water:0.1% TFA in acetonitrile; [B] (50:50), 0.1%TFA in water:0.1% TFA in acetonitrile
gradient: 0-100% B in 65 min
flow rate: 1 mL/min
column temp.: 30 °C
detector: UV, 215 nm
injection: 50μL carboxymethylated apohemoglobin tryptic digest in 50 mM ammonium bicarbonate
suitability
application for HPLC
application(s)
life science and biopharma
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