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G001431

HPLC Analysis of Organic Acids on Discovery® C18

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Discovery C18, 5 cm × 4.6 mm I.D., 5 μm particles (504947)
mobile phase: [A] 0.1% TFA in [B] water:methanol; (60:40, A:B)
flow rate: 2.0 mL/min
column temp.: 20 °C
detector: UV, 254 nm
injection: 10 μL

suitability

application for HPLC

application(s)

chemicals and industrial polymers
food and beverages
vitamins, nutraceuticals, and natural products

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Homovanillic acid
    p-Toluic acid
    Sorbic acid
    Salicylic acid

analytical column

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Description
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