843153

TOYOPEARL^® Butyl-650S Bulk Media

phase C4 (butyl), bottle of 25 mL, 35 μm particle size

• Synonym(s): TOYOPEARL^® Butyl-650 HIC Bulk Media
• UNSPSC Code: 41115700

Properties

• description: Butyl-650S
• product line: TOYOPEARL^®
• form: slurry
• packaging: bottle of 25 mL
• manufacturer/tradename: Tosoh 43153
• parameter: 3 bar max. pressure
• technique(s): LPLC: suitable
• matrix: hydroxylated methacrylic polymer
• matrix active group: C4 (butyl) phase
• particle size: 35 μm
• operating pH: 1-13
• capacity: 40 g/L±10 adsorption capacity (lysozyme)
• separation technique: hydrophobic interaction (HIC)

Description

General description

TOYOPEARL^® HIC resins are hydrophobic interaction chromatography resins that offer the following advantages: strong affinity for water-soluble proteins, high recovery of mass and activity, high sample capacity up to 2-4 times that of gel media, fluctuating salt concentrations will not change the bed volume, mechanical stability to 7kg/cm squared (7 bar/100psi), stability at a wide pH range (2-12), clean in place with 0.5M NaOH, and autoclavable. The exclusion limit of 5x10⁶ Da and large pore size, 1000Å, enables these packings to separate very large proteins by a hydrophobic interaction mechanism, without size exclusion effects.

TOYOPEARL^® HIC resins for hydrophobic interaction chromatography offer these advantages:

• Strong affinity for water-soluble proteins
• High recovery of mass and activity
• High sample capacity: up to 2-4 times that of gel media
• Fluctuating salt concentrations will not change bed volume
• Mechanical stability to 7kg/cm² (7 bar/100psi)
• Stable at wide pH range: 2-12
• Clean in place with 0.5M NaOH
• Autoclavable

     
TOYOPEARL media offer high yield recovery of proteins, using various aqueous eluants. The exclusion limit of 5x10⁶ Da and large pore size, 1000Å, enables these packings to separate very large proteins by a hydrophobic interaction mechanism, without size exclusion effects. Packed as 100mL bottle.

Specifications

Clean in place with 0.5 M NaOH or 0.1 M HCl.

Physical form

72% (v/v) slurry in 20% (v/v) ethanol

Other Notes

Toyopearl Butyl-650 resin has the second highest hydrophobicity of the HIC ligands offered by TBL. Its pore size is the largest of our three butyl resins. Primary applications are for the separation of proteins, their isoforms, and aggregate removal. It is typically used in interresinte purification and polishing steps

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Legal Information

Toyopearl is a registered trademark of Tosoh Corporation

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P210 - P233 - P240 - P241 - P242 - P243
• Hazard Classifications: Flam. Liq. 3
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 3
• Flash Point(F): 104.0 °F - closed cup
• Flash Point(C): 40 °C - closed cup