ZRB2362

Anti-p-PKCγ (Thr514) Antibody, clone 4F20 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): EC:2.7.11.13, PKC-gamma, Protein kinase C gamma type
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: recombinant monoclonal
• description: clone 4F20 ; recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 78.4 kDa; observed mol wt ~78 kDa
• purified by: using Protein A
• species reactivity: mouse, human, rat
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): immunohistochemistry: suitable; inhibition assay: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal half
• Protein ID accession no.: NP_002730
• UniProt accession no.: P05129
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... PRKCG(5582)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 4F20 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects PKC phosphorylated on threonine 514.

Immunogen

KLH-conjugated linear peptide corresponding to 14 amino acids surrounding phosphothreonine 514 from human PKC .

Application

Quality Control Testing

Evaluated by Western Blotting in Human brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Phospho-PKC (Thr514) in Human brain tissue lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Phospho PKC (Thr514) in Mouse brain tissue lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Phospho PKC (Thr514) in Human brain and Rat brain tissue sections.

Peptide Inhibition Assay: Target band detection in a lysate from Human brain tissue lysate was prevented by preblocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Protein kinase C gamma type (UniProt: P05129; also known as EC:2.7.11.13, PKC-gamma) is encoded by the PRKCG (also known as PKCG) gene (Gene ID: 5582) in human. Protein kinase C (PKC), a ubiquitous, calcium-activated, phospholipid dependent serine/threonine kinase that is involved in signal transduction associated with cell proliferation, differentiation, and apoptosis. At least eleven closely related PKC isozymes have been reported that differ in their structure, biochemical properties, tissue distribution, subcellular localization, and substrate specificity. PKC is a calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine kinase that plays diverse roles in neuronal cells and eye tissues. It binds and phosphorylates GRIA4/GLUR4 glutamate receptor and regulates its function by increasing plasma membrane-associated GRIA4 expression. The catalytic domain of PKC contains an ATP-binding site. Its regulatory domain has a Ca2+ binding site. The amino terminal half of the regulatory domain contains two conserved regions, C1 and C2 that play a vital role in the regulation of enzyme activity. The catalytic domain is composed of highly conserved C3 and C4 regions. The C3 region contains the ATP-binding consensus sequence, whereas the C4 region is responsible for protein substrate binding. The protein kinase domain of PKC is localized in amino acids 351-614 and the AGC-kinase C-terminal domain resides in amino acids 615-685. Two isoforms of PKC have been described that are produced by alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Saito, N., and Shirai, Y. (2002). J. Biochem. 132(5); 683-687; Coussens, L., et al. (1986). Science. 233(4766); 859-866).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable