ZMS1211
Anti-Fibrin Antibody, clone 59D8 ZooMAb® Mouse Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Fibrinogen beta chain
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
biological source
mouse
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
recombinant monoclonal
description
clone 59D8
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 55.93 kDa
observed mol wt ~56 kDa
purified by
using protein G
species reactivity
mouse, human
packaging
antibody small pack of 25
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1κ
epitope sequence
N-terminal
Protein ID accession no.
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
Gene Information
human ... FGB(2244)
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 59D8 is a ZooMAb® mouse recombinant monoclonal antibody that specifically detects Fibrin. It targets an epitope within 7 amino acids from the N-terminal region.
Immunogen
KLH-conjugated linear peptide corresponding to 7 amino acids from the N-terminal region of human Fibrinogen β chain.
Application
Quality Control Testing
Evaluated by Western Blotting in Human plasma.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Fibrin in Human plasma.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Fibrin in lysates from HepG2 cells and from Mouse liver tissue..
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Fibrin in Human placenta tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Fibrin in Human kidney and Human placenta tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in Human plasma.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Fibrin in Human plasma.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Fibrin in lysates from HepG2 cells and from Mouse liver tissue..
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Fibrin in Human placenta tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Fibrin in Human kidney and Human placenta tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Fibrinogen beta chain (UniProt: P02675) is encoded by the FGB gene (Gene ID: 2244) in human. Fibrinogen beta chain is synthesized with a signal peptide (aa 1-30), which is subsequently cleaved off to product the mature protein that is further cleaved by thrombin to produce fibrinopeptide B (aa 31-44) and fibrinogen beta chain (45-491). Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers. Fibrin monomers spontaneously aggregate into an insoluble gel, which is then covalently stabilized by Factor XIIIa. Fibrin is shown to retain about 98% of the original covalent structure of fibrinogen. Fibrin clot can be broken down by plasmin by cleave between residues 152-153 and 163-164. A mouse monoclonal version of this clone binds to fibrin even in the presence of human fibrinogen at the concentration found in plasma. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonal. (Ref.: Litvinov, RI., and Weisel, JW (2016). Semin. Thromb. Hemost. 42(4); 333-343; Hui, KY., et al. (1983). Science. 222 (4628); 1129-1132).
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Reconstitution
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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