SRP0690

LKB1(STK11) Protein

recombinant

• UNSPSC Code: 12352202

Properties

• recombinant: expressed in baculovirus infected Sf9 cells
• Assay: 70-95% (SDS-PAGE)
• form: frozen liquid
• mol wt: ~85 kDa
• concentration: 0.2 μg/μL
• accession no.: NM_000455
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... LKB1(6794)

Description

Application

Useful for Western blot and in kinases assays as a downstream substrate for SRC or MEK1 kinases.

Biochem/physiol Actions

Recombinant full-length human LKB1(STK11) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. LKB1(STK11) is a member of the serine/threonine kinase family which regulates cell polarity and functions as a tumor suppressor. STK11 is a critical barrier to pulmonary tumorigenesis which controlling initiation, differentiation, and metastasis. STK11 plays a central role in restricting HSC entry into cell cycle and in broadly maintaining energy homeostasis in hematopoietic cells through a novel metabolic checkpoint ⁽¹⁾. STK11 serves as an essential regulator of HSCs and hematopoiesis, and more generally, points to the critical importance of coupling energy metabolism and stem cell homeostasis ⁽²⁾.

Reconstitution

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Storage and Stability

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable