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SAE0194

Sigma-Aldrich

3xFLAG™ Peptide

≥95% (HPLC), lyophilized powder, purified

Synonym(s):

Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, ddddk peptide, dykddddk peptide

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.43

product name

Purified 3xFLAG® peptide, ≥95% (HPLC), lyophilized powder

biological source

synthetic

Quality Level

200

Assay

≥95% (HPLC)

form

lyophilized powder

technique(s)

protein extraction: suitable

shipped in

dry ice

storage temp.

−20°C

General description

The 3X FLAG Peptide is a synthetic peptide of 23 amino acid residues in length. The Asp-Tyr-Lys-Xaa-Xaa-Asp motif is repeated three times in the peptide. Eight amino acids at the C-terminus make up the classic FLAG sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys).

Application

For use in competitive elution by affinity chromatography of 3X FLAG fusion proteins either from the ANTI-FLAG M2 monoclonal antibody (F1804) in solution, or bound to agarose on the ANTI-FLAG M2 agarose affinity gel (A2220).
For elution under gentle, mild conditions of 3X FLAG fusion proteins from the ANTI-FLAG M2 affinity gels.

(Note: the 1X FLAG Peptide (F3290) will not elute 3X FLAG fusion proteins from ANTI-FLAG affinity gels.)

Legal Information

3xFLAG is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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