S4942
SYPRO® Ruby Protein Gel Stain
Synonym(s):
SYPRO® dye, protein gel stain
About This Item
Recommended Products
shelf life
≥6 mo. (when stored at room temperature and protected from light)
Quality Level
technique(s)
protein staining: suitable
fluorescence
λex 280,450 nm; λem 610 nm
General description
- uses a simple staining protocol, with no possibility of overstaining
- delivers a linear quantitation range of over three orders of magnitude
- shows less protein-to-protein variability
- stains glycoproteins, lipoproteins, calcium-binding proteins, fibrillar proteins, and other difficult-to-stain proteins
- will not stain extraneous nucleic acids
- does not interfere with subsequent analysis of proteins by Edman-based sequencing or mass spectrometry
Application
Caution
Legal Information
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Certificates of Analysis (COA)
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Articles
To meet the great diversity of protein analysis needs, Sigma offers a wide selection of protein visualization (staining) reagents. EZBlue™ and ProteoSilver™, designed specifically for proteomics, also perform impressively in traditional PAGE formats.
The possible causes and potential remedies for challenges encountered during preparation of samples for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and optimizing electrophoresis conditions.
Protocols
Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.
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