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R8653

Sigma-Aldrich

Anti-ROCK-2 antibody produced in rabbit

~2 mg/mL, affinity isolated antibody, buffered aqueous solution

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About This Item

MDL number:
MFCD07370479
UNSPSC Code:
12352203
NACRES:
NA.44

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 160 kDa

species reactivity

human, mouse

concentration

~2 mg/mL

technique(s)

immunoprecipitation (IP): 25-50 μg using Jurkat cell lysate
microarray: suitable
western blot: 2-4 μg/mL using whole cell extract of human acute T cell leukemia Jurkat cell line and of mouse fibroblasts NIH3T3 cell line.

UniProt accession no.

O75116

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ROCK2(9475)
mouse ... Rock2(19878)
rat ... Rock2(25537)

General description

ROCK2 is a serine/threonine kinase that regulates actin cytoskeletal framework. Absence of ROCK2 alleviates atherosclerosis, whereas its overexpression has been associated with hepatocellular carcinoma. Genetic mutations in ROCK2 has been linked to the risk of Behcet′s disease . Anti-ROCK-2 recognizes human and mouse ROCK-2 (160 kDa).

Immunogen

The sequence is highly conserved in Xenopus ROCK-2.
synthetic peptide corresponding to amino acids 1371-1388 located at the C-terminus of human ROCK-2, conjugated to KLH. This sequence is identical in rat, mouse and bovine ROCK-2.

Application

The antibody is suitable for use in immunoprecipitation (25-50 μg using Jurkat cell lysate), microarray and western blot (2-4 μg/mL using whole cell extract of human acute T cell leukemia Jurkat cell line and of mouse fibroblasts NIH3T3 cell line). The antibody may also be used for immunofluorescence (3-6 μg/ml using NIH3T3 cell line).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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ROCK2 Deficiency in Bone Marrow-Derived Cells Leads to Increased Cholesterol Efflux and Decreased Atherosclerosis.
Zhou, Q., et al.
Circulation, doi: 10-doi: 10 (2012)
Wei Chen et al.
Scientific reports, 8(1), 16636-16636 (2018-11-11)
Rho-associated coiled-coil kinase (ROCK)2 targeting down-regulates autoimmune responses in animal models and patients, however the underlying molecular mechanism is still an enigma. We report that ROCK2 binds phosphorylated-STAT3 and its kinase activity controls the formation of ROCK2/STAT3/JAK2 complex and optimal
Association between Rho-kinase (ROCK2) gene polymorphisms and Behcet's disease.
Oguz, E., et al.
Translational Research, doi: 10-doi: 10 (2012)
Tiande Liu et al.
Experimental cell research, 318(16), 1994-2003 (2012-06-19)
Rho-associated coiled-coil containing protein kinase 2 (Rock2) belongs to a family of serine/threonine kinases which are actived via interaction with Rho GTPases. Recently, overexpression of Rock2 has been demonstrated in human hepatocellular carcinoma (HCC), but the potential role of Rock2
He-Song Jiang et al.
International journal of molecular medicine, 36(3), 801-807 (2015-07-17)
The transformation of tunica albuginea-derived fibroblasts (TAFs) into myofibroblasts plays an important role in the pathological progress of Peyronie's disease (PD). However, no treatment which addresses this transformation is currently available. Estrogen has been shown to inhibit the progression of

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