R8529

Monoclonal Anti-RAL-A antibody produced in mouse

~2 mg/mL, clone RalA5F9.2, purified immunoglobulin, buffered aqueous solution

• MDL number: MFCD08705597
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: RalA5F9.2, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 25 kDa
• species reactivity: monkey, mouse, human, rat
• concentration: ~2 mg/mL
• technique(s): immunocytochemistry: 5-10 μg/mL using HeLa cells; indirect ELISA: suitable; microarray: suitable; western blot: 1-2 μg/mL using rat brain extract
• isotype: IgG1
• UniProt accession no.: P11233
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... RALA(5898) ; mouse ... Rala(56044) ; rat ... Rala(81757)

Description

General description

Monoclonal Anti-Ral-A (mouse IgG1 isotype) is derived from the hybridoma RalA5F9.2 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide. Ras-related protein Ral-A is a member of the Ras-like guanosine-5′-triphosphate (GTP) binding protein family. Ral-A protein is expressed in the islets of the pancreas, insulin-secreting cell lines and tissues during embryogenesis. The RALA gene is located on the human chromosome at 7p14.1.

Specificity

Monoclonal Anti-Ral-A recognizes human, monkey, rat and mouse Ral-A, ~25 kDa.

Immunogen

synthetic peptide corresponding to amino-acids 189-203 of human Ral-A, conjugated to KLH.

Application

Monoclonal Anti-RAL-A antibody produced in mouse may be used in:

• immunoblotting
• immunocytochemistry
• enzyme-linked immunosorbent assay (ELISA)

Biochem/physiol Actions

Ras-related protein Ral-A can bind guanosine triphosphate (GTP) or guanosine diphosphate (GDP). It is known to possess a low intrinsic GTPase activity. Ral-A protein is involved in regulating the insulin secretion of pancreatic β-cells. This protein is also involved in membrane trafficking, oncogenic transformation and proliferation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For extended storage, freeze at –20 °C in working aliquots. For continuous use, store at 2-8 °C for up to one month. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves