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P4557

Sigma-Aldrich

Phenol solution

BioReagent, Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, for molecular biology

Synonym(s):

Tris-equilabrated Phenol

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About This Item

Empirical Formula (Hill Notation):
C6H6O
CAS Number:
108-95-2
Molecular Weight:
94.11
Beilstein:
6428463
EC Number:
203-632-7
MDL number:
MFCD00002143
UNSPSC Code:
12352104
PubChem Substance ID:
57654529
NACRES:
NA.52

grade

for molecular biology

Quality Level

200

product line

BioReagent

Assay

≥99.0% (GC)

form

liquid

pH

7.7-8.1 (25 °C)

storage temp.

2-8°C

SMILES string

Oc1ccccc1

InChI

1S/C6H6O/c7-6-4-2-1-3-5-6/h1-5,7H

InChI key

ISWSIDIOOBJBQZ-UHFFFAOYSA-N

Gene Information

human ... GABRA1(2554)

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General description

A tris-equilibrated phenol solution is a clear liquid capped with a layer of Tris buffer. Phenol solutions can be used for a wide variety of molecular biology applications. Organic compounds like phenols have an aromatic carbocyclic ring with a hydroxyl group attached directly to it. The common name for monohydroxybenzene is phenol.

Application

Phenol solution has been used:

  • as a component of the digestion reaction mix to prepare RNase-free plasmids for in vitro translation in a rabbit reticulocyte lysate
  • for extraction of DNA from Arabidopsis thaliana Rosette leaves
  • in the extraction of DNA from blood on preservation paper by standard phenol-chloroform protocol
  • as a component of extraction buffer to extract RNA from fruit pulp
  • for the extraction of protein from roots
  • for the isolation of fecal DNA
  • for the extraction of caged RNA

Biochem/physiol Actions

The phenol-chloroform extraction method is widely employed to extract nucleic acids. Even though phenol does not completely inhibit RNase activity, its unique characteristic makes it beneficial for the isolation of nucleic acids when used in combination with chloroform and alcohol.

Components

  • 1 bottle Tris-equilabrated Phenol (product number P4557)
  • Equilibration Buffer (product B5658)

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

Target Organs

Nervous system,Kidney,Liver,Skin

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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