MAK313
Adipolysis Assay Kit
sufficient for 200 colorimetric or fluorometric tests
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
application(s)
pharmaceutical
detection method
colorimetric
fluorometric
relevant disease(s)
endocrinological disorders, diabetes; obesity; cardiovascular diseases; cancer
storage temp.
−20°C
General description
Obesity is a chronic condition that develops from storage of excessive energy in the form of adipose tissue. The resulting adiposity presents a high risk factor for diseases such as type 2 diabetes, cardiovascular diseases, and cancer. Adipolysis or lipolysis is a highly regulated process in fat metabolism, in which triglycerides are broken down into glycerol and free fatty acids. Rapid, robust and accurate procedures for adipolysis quantification in cell culture are very useful in research and drug discovery.
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
Suitable for adipolysis quantification in cell culture.
Principle
This adipolysis assay kit directly measures glycerol released during adipolysis in cultured cells. This homogeneous assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase, and color reactions in one step, resulting in a colorimetric (570 nm)/fluorimetric (λex = 530/ λem = 587 nm) product, proportional to the glycerol present.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral
Storage Class Code
10 - Combustible liquids
Flash Point(F)
188.6 °F
Flash Point(C)
87 °C
Certificates of Analysis (COA)
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Saveta G Mladenova et al.
Frontiers in pharmacology, 12, 707507-707507 (2021-09-07)
Obesity is a persistent and continuously expanding social health concern. Excessive fat mass accumulation is associated with increased risk of chronic diseases including diabetes, atherosclerosis, non-alcoholic steatohepatitis, reproductive dysfunctions and certain types of cancer. Alchemilla monticola Opiz. is a perennial
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