H4149

Monoclonal Anti-Heat Shock Protein 60 antibody produced in mouse

clone LK1, ascites fluid

• MDL number: MFCD02189138
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: LK1, monoclonal
• mol wt: antigen 60 kDa
• contains: 15 mM sodium azide
• species reactivity: rat, human, chicken
• technique(s): electron microscopy: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; indirect ELISA: suitable; western blot: 1:400 using cultured human foreskin fibroblast extract
• isotype: IgG1
• UniProt accession no.: P10809
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... HSPD1(3329) ; rat ... Hspd1(63868)

Description

General description

Heat shock (stress) proteins (HSP) are produced by prokaryotic and eukaryotic cells, and are among the most conserved molecules in phylogeny. HSP-60 is a member of HSP family with a uniquely high level of sequence conservation.

Monoclonal Anti-Heat Shock Protein 60 (mouse IgG1 isotype) is derived from the LK1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant human heat diseases.

Specificity

The antibody recognizes an epitope located between amino acid residues 383-447 of the human HSP 60. It is not cross-reactive with the bacterial counterpart (E. coli), or with helminth and spinach HSP60. The antibody reacts with constitutive and inducible HSP60. It demonstrates a raised level of staining in immunohistochemistry of synovial membranes taken from patients with juvenile chronic arthritis.

Immunogen

recombinant human heat shock protein 60 (HSP60)

Application

Monoclonal Anti-Heat Shock Protein 60 has been used in the following techniques:

• Enzyme linked immunosorbent assay (ELISA)
• Immunoblotting
• Immunocytochemistry
• Immunohistochemistry
• Immunoelectron microscopy
• Double immunofluorescence

Biochem/physiol Actions

A wide variety of environmental perturbations, such as a sudden increase in temperature, induce cells to rapidly synthesize a group of polypeptides known as the heat shock (stress) proteins. HSP-60 is considered to be a potential antigen in a number of autoimmune diseases. Abnormal immune reactivity involving shock protein 60 (HSP60) has also been implicated in the pathogenesis of schizophrenia. This monoclonal antibody enables the differentiation between the HSP60 of mammalian and bacterial origin.

Abnormal immune reactivity involving HSP60 has also been implicated in the pathogenesis of schizophrenia.

Physical form

Monoclonal Anti-Heat Shock Protein 60 is provided as ascites fluid with 0.1% sodium azide as a preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable