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form
liquid
packaging
pkg of 1 kit
manufacturer/tradename
Cytiva RPN3682
shipped in
wet ice
storage temp.
2-8°C
General description
AlkPhos Direct Labeling and Detection Systems are based on the rapid, direct labeling of DNA or RNA probes with thermostable alkaline phosphatase. CDP-Star detection reagent allows for chemiluminescent detection of Alkaline Phosphatase labelled nucleic acids.
Sufficient reagent for 2500cm² of membrane. Can be used in conjunction with AlkPhos Direct Labeling Module (RPN3680), Amersham AlkPhos Direct Hybridization Buffer (RPN3688) and Hybond/ Nytran membranes.
Each system includes the following reagents, sufficient for 25 or 50 labeling reactions and detection of 2500 cm2 or 5000 cm2 membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer, blocking agent, CDP-Star or ECF™ detection reagents.
Sufficient reagent for 2500cm² of membrane. Can be used in conjunction with AlkPhos Direct Labeling Module (RPN3680), Amersham AlkPhos Direct Hybridization Buffer (RPN3688) and Hybond/ Nytran membranes.
Each system includes the following reagents, sufficient for 25 or 50 labeling reactions and detection of 2500 cm2 or 5000 cm2 membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer, blocking agent, CDP-Star or ECF™ detection reagents.
Application
CDP-Star® has been used to label and quantify genomic DNA by Southern blotting.
Features and Benefits
- Probes are labelled during a 30 minute reaction, resulting in probes that can be used without purification.
- Hybridization stringency can be controlled by temperature as well as salt concentration. Detection is rapid, taking only 30 minutes from completion of hybridization to application of detection reagent.
- Detection is rapid, taking only 30 minutes from completion of hybridization to application of detection reagent.
Storage and Stability
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
CDP-Star is a registered trademark of Tropix, Inc.
ECF is a trademark of Cytiva
Signal Word
Danger
Hazard Statements
Precautionary Statements
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Daniel de Melo Pereira et al.
Frontiers in bioengineering and biotechnology, 8, 554565-554565 (2020-11-17)
Biomineralized collagen with intrafibrillar calcium phosphate mineral provides an excellent mimic of the composition and structure of the extracellular matrix of bone, from nano- to micro-scale. Scaffolds prepared from this material have the potential to become the next-generation of synthetic
Articles
Background and protocols describing the various methods used by molecular biologists to detect samples of protein or nucleic acids bound to membranes.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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