GE29-0485-81
HiTrap® Protein G HP
Cytiva 29-0485-81, pack of 1 mL
About This Item
Recommended Products
ligand
recombinant protein G lacking albumin-binding region
packaging
pack of 1 mL
manufacturer/tradename
Cytiva 29-0485-81
availability
not available in North America
storage condition
(20% Ehtanol)
(20% Ethanol)
parameter
flow rate
42 psi
bed size
7 mm × 25 mm
bed volume
1 mL
column I.D.
7 mm
matrix
highly cross-linked 6% agarose
avg. part. size
34 μm
cleaning
2-9
working range
3-9
capacity
>1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.)
25 mg binding capacity(human IgG/column)
storage temp.
2-8°C
General description
Features and Benefits
34 μm bead size for high performance, high-resolution purifications.
Binds broad range of different species and subclasses of IgG.
Simple and proven method giving reproducible results.
Designed for use with syringe, peristalic pump, or chromatography system.
Storage and Stability
Other Notes
Legal Information
related product
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
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Articles
Antibodies are needed for research and industrial purposes in different quantities, from microgram to kilogram scale. It is important to design and use a purification method that will yield protein of a quality and quantity that is adequate for the particular application.
Purify monoclonal or polyclonal IgG from serum, cell culture supernatant or ascitic fluid using the HiTrap Protein G HP from Cytiva, an affinity-exclusion chromatography product containing Sepharose-immobilized Protein G.
This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.
This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from Cytiva.
Protocols
This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap® Protein G HP from Cytiva for the capture step.
This page shows general instructions for affinity purification using HiTrap columns from GE Healthcare.
This page shows how to prepare samples for purification with affinity chromatography.
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