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GE17-5112-01

HiTrap® Streptavidin High Performance

Cytiva 17-5112-01, pack of 5 × 1 mL

Synonym(s):

HiTrap Streptavidin, Streptavidin High Performance

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About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

packaging

pack of 5 × 1 mL

manufacturer/tradename

Cytiva 17-5112-01

storage condition

(20% Ethanol)
 (20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8)

parameter

42 psi

bed size

7 mm × 25 mm

bed volume

1 mL

column I.D.

7 mm

matrix

highly cross-linked 6% agarose

avg. part. size

34 μm

cleaning

2-10.5

working range

4-9

capacity

binding capacity(>300 nmol biotin/ml medium 6 mg biotinylated BSA/ml medium)

storage temp.

2-8°C

General description

HiTrap® Streptavidin HP is a column prepacked with Streptavidin Sepharose™ High Performance for purification of biotinylated proteins and biomolecules.

Application

The prepacked Streptavidin Sepharose™ High Performance medium ensures reproducibility, high performance, and excellent physical and chemical stability. Streptavidin Sepharose™ High Performance is also available in lab packs, using the same purified streptavidin isolated from Streptomyces avidinii bound to Sepharose™ High Performance.

Features and Benefits

  • Convenient, fast, reliable purification of biotinylated compounds.
  • The strong interactions of biotin and streptavidin, as well as the somewhat weaker interactions of 2-iminobiotin and streptavidin are utilized.
  • The column can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA® design or FPLC™ System.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

FPLC is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Performing a Separation of Biotin and Biotinylated Substances with HiTrap Streptavidin HP and Streptavidin Sepharose High Performance

How to separate biotin and biotinylated substances by affinity chromatography using HiTrap Streptavidin HP and Streptavidin Sepharose High Performance products from GE Healthcare.

Purification or Removal of Biotin and Biotinylated Substances

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Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

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