GE17-0043-02
Sephadex® G-50
Medium, Cytiva, pack of 500 g
About This Item
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feature
autoclavable In wet form (pH 7.0) at 120°C for 30 min
packaging
pack of 500 g
manufacturer/tradename
Cytiva
swelling
1 g swells to 9-11 mL
particle size
100-300 μm (wet)
50-150 μm (dry)
cleaning in place
2-13
working range
2-10
InChI
1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2
InChI key
FZWBNHMXJMCXLU-UHFFFAOYSA-N
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General description
Sephadex® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-50 is available in 4 different particle sizes (Course, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Cource and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.
Features and Benefits
- Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
- Suitable for purification of DNA from small molecules by gel filtration
- Larger bead size for large scale group separations where high flow rates and low operating pressures are required.
- Classic gel filtration medium.
Storage and Stability
Analysis Note
Legal Information
Storage Class Code
13 - Non Combustible Solids
Certificates of Analysis (COA)
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Protocols
This page shows how to perform a separation with a Sephadex column from GE Healthcare which are Size Exclusion Chromatography media consisting of an epichlorohydrin cross-linked dextran.
Related Content
Sephadex® from Cytiva and Darcy’s law in Size Exclusion Chromatography
Separation options for Size Exclusion Chromatography with Sephadex from Cytiva.
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