G5035

Granulocyte-Macrophage Colony-Stimulating Factor human

GM-CSF, recombinant, expressed in E. coli, suitable for cell culture

• Synonym(s): GM-CSF human
• MDL number: MFCD00166611
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• biological source: human
• Quality Level: 200
• recombinant: expressed in E. coli
• Assay: ≥98% (SDS-PAGE)
• form: lyophilized powder
• potency: ≤0.1 ng/mL EC₅₀
• mol wt: 14.6 kDa
• packaging: pkg of 4X5 μg; pkg of 5 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1.000 EU/μg
• color: white
• solubility: water: soluble 0.010 mL, clear, colorless
• UniProt accession no.: P04141
• storage temp.: −20°C
• Gene Information: human ... CSF2(1437) , CSF2(1437)

Description

General description

Package size based on protein content

Biochem/physiol Actions

Granulocyte-macrophage colony stimulating factor (GM-CSF) is a growth and differentiation factor for cells in the granulocyte, macrophage and eosinophil lineage. GM-CSF stimulates colony formation from pluripotential progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. It also stimulates growth in some epithelial cells and osteoclasts. GM-CSF is produced by a variety of cell types (monocytes, endothelial cells, T-cells, fibroblasts, mitogen-stimulated B-cells, and LPS-stimulated macrophages). GM-CSF is secreted as a single chain glycoprotein containing 128 amino acids for human with a conserved disulfide bond. Human and murine GM-CSF share approx. 54% sequence homology and do not cross-react in bioactivity.

Physical form

Lyophilized from a 0.2 μm filtered, 10 mM sodium citrate, pH 3.5 solution.

Analysis Note

The proliferative activity of human GM-CSF is tested in culture using human TF-1 cells.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves