D9655

pBR322 Hae III Digest

for DNA electrophoresis

• UNSPSC Code: 12352202
• NACRES: NA.25

Properties

• form: liquid
• usage: 1000 uses (0.5 mg size); 200 uses (0.1 mg size)
• suitability: suitable for electrophoresis (DNA)
• storage temp.: −20°C

Description

General description

Sigma′s pBR322 Hae III Digest contains 22 fragments from 8 - 587 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Application

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

Components

Sigma′s pBR322 Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Other Notes

Contains 22 fragments, 8–587 bp

For optimal resolution, the recommended agarose gel concentration is 2.5.

Preparation Note

Plasmid pBR322 DNA was digested with Hae III to completion, then purified by phenol extraction.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable