CWDS-IF594

ColorWheel^® Antibody-Ready iFluor 594 Dye

for use with ColorWheel^® Antibody (Required, Sold Separately)

• Synonym(s): iFluor 594
• UNSPSC Code: 12352203

Properties

• product line: ColorWheel^®
• Quality Level: 200
• form: lyophilized
• purified by: ion-exchange chromatography
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• technique(s): flow cytometry: suitable
• fluorescence: λ_ex 588 nm; λ_em 604 nm
• compatibility: for use with ColorWheel^® Antibody (Required, Sold Separately)
• greener alternative category: , Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

iFluor 594 is optimized for labeling antibodies and proteins. It is a pH-independent dye that works over a wide range of pH (3 to 11). It displays an excitation max at 588 nm and an emission max at 604 nm. Its spectral properties are similar to Texas Red^® and ALEXA FLUOR^™ 594. However, compared to Texas Red^®, it is easier to conjugate with R-Phycoerythrin (RPE) with higher conjugation yield and the resulting RPE-iFluor 594 tandem dye offers better FRET efficiency. It exhibits decent stability shows good reactivity and selectivity with amino groups.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Specificity

ColorWheel Antibody-Ready iFluor 594 Dye is designed for use with ColorWheel Dye-Ready antibodies in Flow cytometry.

Application

Flow Cytometry: Excitation: 588 nm, Emission: 604 nm.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Compatibility

Requires ColorWheel^® Antibody (Sold Separately)

Physical form

CWDS-IF594-25UL Set Components

CWDS-IF594-25UL - ColorWheel^® Antibody-Ready iFluor 594 Dye Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

CWD-FINISH-25UL - ColorWheel^® Finishing Buffer Lyophilized from PBS. Contains no biocide or preservatives, such as azide.

Reconstitution

Reconstitute each vial by adding 25 μL PBS.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Texas Red is a registered trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3