VA-ES-BJ

94092303

• UNSPSC Code: 41106514

Properties

• biological source: human skin (bone marrow metastasis)
• growth mode: Adherent
• karyotype: 66-68 Aneuploidic, pentasomy #7, multiple trisomy
• morphology: Epithelial
• products: Granulocyte Macrophage Colony Stimulating Factor (GM-CSF)
• receptors: Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), AE, AE3, Fas, epithelial membrane antigen (EMA), Vimentin, P53
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): metastasis
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human skin epithelioid sarcoma

Cell Line Description

Spontaneously transformed cells from a male patient with epithelioid sarcoma derived from bone marrow metastasis. Cells grow very rapidly and secrete GM-CSF. They have been shown to express AE-1 and AE-3 cytokeratins, epithelial membrane antigen, p53, human Fas and vimentin. VA-ES-BJ cells are sensitive to oxidising agents, busulfaxamine and protoporphyrin IX. Epithelial, spindle and giant cells.

Application

Study of tumours. Tumourigenic in nude mice.

Culture Medium

DMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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