8305C

94090183

• UNSPSC Code: 41106514

Properties

• biological source: human thyroid
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human thyroid anaplastic carcinoma, undifferentiated.

Cell Line Description

Established from undifferentiated thyroid carcinomas of a 67 year old female patient. Pathologically the carcinoma tissue contained residual well differentiated components suggesting well differentiated to undifferentiated carcinoma progression. Tumour suppresser genes p53, Rb, APC and MCC were analysed and sequence analysis confirmed a C:G to T:A transition at the first base of p53 gene codon 273. Loss of heterozygosity of tumour suppresser genes was not observed.

Application

As p53 mutations associate with undifferentiated carcinoma but not well differentiated carcinoma during multistep carcinogenesis of the thyroid, these cell lines should prove useful for research into the role of gene mutation in malignant transformation.

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 9,12
D13S317: 9
D16S539: 10,11
D5S818: 10,13
D7S820: 8,10
THO1: 6,7
TPOX: 8
vWA: 14,16

Culture Medium

EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Doubling time is 43 hours. Saturation density at confluency is 5.7x10,000 cells/cm².

Other Notes

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