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biological source
rat bone
growth mode
Adherent
karyotype
Not specified
morphology
Not specified
products
Not specified
receptors
Cytosol receptors for the bone resorbing steroid hormone 1,25 -(OH)2 D3.
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Rat osteosarcoma
Cell Line Description
A cloned derivative of a transplantable rat osteogenic sarcoma. The cells retain many of the characteristics of the tumour including responsiveness to parathyroid hormone (PTH) and prostaglandins.
Application
Tumourigenicity and hormone studies
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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