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biological source
insect ovary
growth mode
Adherent
karyotype
Not specified
morphology
Spherical
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Spodoptera frugiperda pupal ovarian tissue, serum-free
Cell Line Description
This cell line is a derivative of the Sf21 cell line which has been adapted to TiterHigh Sf Insect Medium (Sigma I5408). TiterHigh Sf Insect Medium is an animal component-free, protein-free formulation designed specifically for the Sf21 (Spodoptera frugiperda) insect cell line. The cell line Sf21 is derived from pupal ovarian tissue of spodoptera frugiperda. The Sf21 cell line is highly susceptible to Baculovirus infection and is used in the production of protein products genetically manipulated into Baculovirus vector systems.
Application
Protein expression using the Baculovirus vector system, serum-free
Culture Medium
TiterHigh™ Sf Insect Medium (Sigma I5408) no supplements.
Subculture Routine
Maintain cultures between 3x100,000 to 1x1,000,000 cells/ml; 27°C. Gently resuspend cells in old culture medium by dislodging the cells by pipetting, or gentle agitation, and resuspend in fresh medium. Cells should stick to the culture flask. Freeze in 7.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Legal Information
TiterHigh is a trademark of Sigma-Aldrich Co. LLC
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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