C4899
Carnitine Acetyltransferase from pigeon breast muscle
ammonium sulfate suspension, ≥50 units/mg protein
Synonym(s):
CATC, Carnitine acetyltransferase, Acetyl-Co-A:carnitine-O-acetyltransferase, CrAT
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Recommended Products
biological source
pigeon breast
Quality Level
form
ammonium sulfate suspension
specific activity
≥50 units/mg protein
concentration
≥0.4 mg/mL
technique(s)
cell based assay: suitable
Protein ID accession no.
UniProt accession no.
storage temp.
2-8°C
Gene Information
pigeon ... CRAT(102084317)
Looking for similar products? Visit Product Comparison Guide
General description
Research area: Cell Signaling
Application
Carnitine Acetyltransferase from pigeon breast muscle has been used in enzymatic assays.
Biochem/physiol Actions
Carnitine acyltransferases (CrAT) are enzymes that contribute to the reversible conversion of acetyl-CoA and carnitine into acetylcarnitine and free CoA. This enzymatic process plays a vital role in the energy metabolism of eukaryotes by promoting the β-oxidation of fatty acids. CrAT-mediated acetyl carnitine production and efflux help maintain a balance between acetyl-CoA and acetyl carnitine in the mitochondria, regenerate free CoA, and alleviate the product inhibition of pyruvate dehydrogenase (PDH), which is a key enzyme in glucose oxidation. This process promotes glucose homeostasis and helps maintain optimal cellular energy metabolism. Carnitine acetyltransferase activity also aids in the progression of the cell cycle from G1 to S phase. carnitine acetyltransferase deficiency also leads to the development of various neurological disorders including Alzheimer′s disease, ataxic encephalopathy, and several vascular diseases.
Carnitine acetyltransferase maintains the cellular and mitochondrial levels of acetyl-CoA, a key cofactor required for oxidative metabolism, by catalyzing an equilibrium between acetyl-CoA and acetyl-L-carnitine, a storage form of activated acetate. Carnitine acetyltransferase also maintains the pool of acetyl-CoA required for neuronal and nonneuronal acetylcholine production.
Unit Definition
One unit will convert 1.0 μmole of acetyl-L-carnitine and CoA to L-carnitine and acetyl-CoA per min at pH 8.0 at 25 °C.
Physical form
Crystalline suspension in 3.2 M (NH4)2SO4 solution, 50 mM potassium phosphate, 1 mM dithiothreitol, pH 7.0
Analysis Note
Protein determined by biuret.
inhibitor
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Rui Wu et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(9), 3259-3263 (2012-02-14)
Phenotypic plasticity occurs prevalently and plays a vital role in adaptive evolution. However, the underlying molecular mechanisms responsible for the expression of alternate phenotypes remain unknown. Here, a density-dependent phase polyphenism of Locusta migratoria was used as the study model
Alfred Lohninger et al.
Gynakologisch-geburtshilfliche Rundschau, 49(4), 230-235 (2009-01-01)
Increased plasma free fatty acid (FFA) levels are a feature of insulin resistance and type 2 diabetes. The aim of the present study was to assess the effect of L-carnitine supplementation on plasma lipids and the expression of enzymes in
Marie A Schroeder et al.
Circulation. Cardiovascular imaging, 5(2), 201-209 (2012-01-13)
Carnitine acetyltransferase catalyzes the reversible conversion of acetyl-coenzyme A (CoA) into acetylcarnitine. The aim of this study was to use the metabolic tracer hyperpolarized [2-(13)C]pyruvate with magnetic resonance spectroscopy to determine whether carnitine acetyltransferase facilitates carbohydrate oxidation in the heart.
Ann T Hanna-Mitchell et al.
Life sciences, 80(24-25), 2298-2302 (2007-03-17)
Non-neuronal release of acetylcholine (ACh) has been proposed to play a role in urinary bladder function. These studies investigated the expression and function of the non-neuronal cholinergic system in cultured urothelial cells isolated from the rat urinary bladder. Our findings
Kristaps Jaudzems et al.
Journal of enzyme inhibition and medicinal chemistry, 24(6), 1269-1275 (2009-11-17)
Carnitine acetyltransferase (CrAT; EC 2.3.1.7) catalyzes the reversible transfer of acetyl groups between acetyl-coenzyme A (acetyl-CoA) and L-carnitine; it also regulates the cellular pool of CoA and the availability of activated acetyl groups. In this study, biochemical measurements, saturation transfer
Articles
Instructions for working with enzymes supplied as ammonium sulfate suspensions
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service