BPQ24
BioPORTER® QuikEase™ Protein Delivery Kit
for intracellular delivery
Synonym(s):
BioPORTER Kit, Protein Delivery Kit
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.26
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General description
Contains 24 individual tubes of BioPORTER reagent
Application
BioPORTER® QuikEase™ Protein Delivery Kit has been used to deliver active granzyme B tagged with FITC into mouse neuroblastoma cell lines. Protein concentrations used for delivery were 6μg/ml which when stained for FITC-GrB yielded a 90% uptake rate.
BioPORTER® QuikEase™ Protein Delivery Kit has been used:
- for the delivery of single-chain antibody variable fragment (scFv) into HeLa cells
- for the transduction of QBI-293 cells with human Tau protein
- for the delivery of human granzyme B (GrB) in human cervical cancer cells
- for the delivery of epidermal fatty acid-binding protein (E-FABP) into nerve growth factor-differentiated PC12 cells (NGFDPC12)
The BioPORTER® QuikEase™ Protein Delivery Kit is the latest innovation in macromolecule delivery technology. It is a unique lipid-based formulation that allows for the intracellular delivery of proteins, peptides, and bioactive molecules such as enzymes and antibodies, into a broad range of cell types. The BioPORTER QuikEase kits contain individual tubes of the BioPORTER Reagent in a convenient, ready-to-use, single reaction format. Each tube contains sufficient material of the lyophilized BioPORTER Reagent to perform 1 reaction in a 6-well plate (or 35 mm dish), 4 reactions in a 24-well plate (or 16 mm wells), and 10 reactions in a 96-well plate.
Legal Information
BioPorter is a registered trademark of Gene Therapy Systems, Inc.
QuikEase is a trademark of Gene Therapy Systems, Inc.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Seeding of normal Tau by pathological Tau conformers drives pathogenesis of Alzheimer-like tangles
Guo JL and Lee VMY
The Journal of Biological Chemistry, 286(17), 15317-15331 (2011)
E. coli expression of a soluble, active single-chain antibody variable fragment containing a nuclear localization signal
Xiong H, et al.
Protein Expression and Purification, 66(2), 172-180 (2009)
Cancer vaccination drives Nanog-dependent evolution of tumor cells towards an immune-resistant and stem-like phenotype
Noh KH, et al.
Cancer Research, canres-ca3758 (2012)
Xianzhi Jiang et al.
Journal of virology, 85(5), 2325-2332 (2010-12-24)
The herpes simplex virus type 1 (HSV-1) latency-associated transcript (LAT) is the only HSV-1 gene transcript abundantly expressed throughout latency. LAT null mutants have a significantly reduced reactivation phenotype. LAT's antiapoptosis activity is the major LAT factor involved in supporting
Jo-Wen Liu et al.
Journal of neurochemistry, 132(1), 85-98 (2014-08-26)
Epidermal fatty acid-binding protein (E-FABP/FABP5/DA11) binds and transport long-chain fatty acids in the cytoplasm and may play a protecting role during neuronal injury. We examined whether E-FABP protects nerve growth factor-differentiated PC12 cells (NGFDPC12 cells) from lipotoxic injury observed after
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