B5655
BCIP®/NBT
SIGMAFAST™, alkaline phosphatase substrate, chromogenic, tablet
Synonym(s):
BCIP/NBT Substrate, BCIP®/NBT Alkaline Phosphatase Substrate
About This Item
Recommended Products
product name
SIGMAFAST™ BCIP®/NBT, tablet
form
tablet
Quality Level
solubility
water: 10 mL, clear, yellow
storage temp.
−20°C
Related Categories
Application
Reconstitution
Legal Information
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
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Articles
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.
Protocols
5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro blue tetrazolium (NBT) is an ideal insoluble substrate for use with alkaline phosphatase. This system produces a blue-purple product. The intense color can be observed visually, is very stable, and will not fade upon exposure to light.
Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.
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