A7888

Bovine Serum Albumin

heat shock fraction, suitable for RIA, pH 5.2, ≥96%

• Synonym(s): Albumin bovine serum, BSA, Bovine albumin
• CAS Number: 9048-46-8
• EC Number: 232-936-2
• MDL number: MFCD00130384
• UNSPSC Code: 12352202
• NACRES: NA.27

Properties

• biological source: bovine
• Quality Level: 200
• grade: RIA grade
• Assay: ≥96%
• form: lyophilized powder
• quality: suitable for RIA
• mol wt: ~66 kDa
• purified by: heat shock fractionation
• packaging: poly bottle of
• origin: USA origin
• technique(s): blood typing: suitable
• loss: ≤5%
• pH: 5.2
• solubility: water: soluble (40 mg/ml)
• suitability: suitable for insulin RIA procedures
• UniProt accession no.: P02769
• foreign activity: BT virus, none detected; VSV virus, none detected
• storage temp.: 2-8°C
• Gene Information: bovine ... ALB(280717)

Description

General description

Bovine serum albumin (BSA) is 66 kDa protein consisting of three domains with two subdomains under each. It is α-helical globular and a non-glycosylated protein.

Application

Bovine Serum Albumin has been used:

• as a blocking agent in enzyme linked immunosorbent assay (ELISA)(154)
• as a component of Dulbecco PBS medium for preantral follicles culture(155)
• as a component of modified Tris-buffered medium (mTBM) for oocyte insemination(156)

Biochem/physiol Actions

Bovine Serum Albumin (BSA) is a transporter for drugs, hormones and fatty acids. It is a blocking agent used in enzyme linked immunosorbent assay (ELISA) for preventing non-specific binding of antigens and antibodies to the microtiter plates. The cysteine and tyrosine residues of BSA have high affinity for metal binding.

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Features and Benefits

• Heat shock fractionated
• Suitable for radioimmunoassay (RIA)

Caution

May yield an insulin blank of up to 0.1 μunit per mg in certain procedures.

Preparation Note

Prepared using heat shock fractionation

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves