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A5852

Sigma-Aldrich

Alkaline Phosphatase Stop Solution

powder, for ELISA

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.83

product name

Alkaline Phosphatase Stop Solution, for ELISA

Quality Level

100

form

powder

solubility

water: 100 mL, clear, colorless

storage temp.

2-8°C

Application

Stop solution for Alkaline Phosphatase Red and Blue microwell substrate solutions. Use 50 μl of stop solution per 200 μl of reaction mix. Alkaline Phosphatase Stop Solution has been used in terminating the alkaline phosphatase and nitrotetrazolium blue chloride (NBT)/5-bromo-4-chloro-3-indolyl phosphate (BCIP) reaction on nitrocellulose membrane.

Reconstitution

Reconstitute with 100 mL of water and mix for 30-60 min.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319,H335

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A screen for over-secretion of proteins by yeast based on a dual component cellular phosphatase and immuno-chromogenic stain for exported bacterial alkaline phosphatase reporter.
Nallaseth FS and Anderson S
Microbial cell factories, 12(1), 36-36 (2013)

Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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