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20966

Sigma-Aldrich

Cesium chloride

BioUltra, for molecular biology, ≥99.5% (AT)

Synonym(s):

CsCl, cesium monochloride

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About This Item

Linear Formula:
CsCl
CAS Number:
7647-17-8
Molecular Weight:
168.36
EC Number:
231-600-2
MDL number:
MFCD00010955
UNSPSC Code:
12352302
PubChem Substance ID:
57647887
NACRES:
NA.25

grade

for molecular biology

Quality Level

200

product line

BioUltra

Assay

≥99.5% (AT)

form

solid

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected
≤0.001% total nitrogen (N)

loss

≤0.1% loss on drying, 110 °C

pH

6.0-7.5 (25 °C, 3 M in H2O)

mp

645 °C (lit.)

solubility

H2O: 3 M at 20 °C, clear, colorless

anion traces

sulfate (SO42-): ≤20 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤50 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

3 M in H2O

UV absorption

λ: 260 nm Amax: 0.02
λ: 280 nm Amax: 0.02

SMILES string

[Cl-].[Cs+]

InChI

1S/ClH.Cs/h1H;/q;+1/p-1

InChI key

AIYUHDOJVYHVIT-UHFFFAOYSA-M

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Biochem/physiol Actions

Cesium chloride (CsCl) can be used for the preparation of electrically conducting glasses. It can also be used to make solutions for the separation of RNA from DNA by density gradient centrifugation.

Other Notes

Cesium chloride gradient centrifugation for phage purification; Isolation of satellite DNA; RNA purification; Buoyant density of DNA - Hoechst 33258 complexes in CsCl gradients.

Pictograms

Health hazard
GHS08

Signal Word

Warning

Hazard Statements

H361f

Hazard Classifications

Repr. 2

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Cooney, C. A. et al.
Methods in Molecular Biology, 2, 21-21 (1984)
Turpen, T. H. and Griffith, O. M.
Biotechnology, 4, 11-11 (1986)
P Karlovsky et al.
Analytical biochemistry, 194(1), 192-197 (1991-04-01)
Buoyant density of DNA in CsCl gradients with Hoechst 33258 (bisbenzimide) was investigated as a function of guanine plus cytosine content of the DNA (%GC; in mole percent). A formula for calculating %GC from the refractive index (nD) of the
Miller, H.
Methods in Enzymology, 152, 159-159 (1987)
Piotr Jurkiewicz et al.
Biochimica et biophysica acta, 1818(3), 609-616 (2011-12-14)
Effects of alkali metal chlorides on the properties of mixed negatively charged lipid bilayers are experimentally measured and numerically simulated. Addition of 20mol% of negatively charged phosphatidylserine to zwitterionic phosphatidylcholine strengthens adsorption of monovalent cations revealing their specificity, in the
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