47670

Formamide

puriss. p.a., ACS reagent, ≥99.5% (GC/T)

• Synonym(s): Amide C₁, Formic amide
• Linear Formula: HCONH₂
• CAS Number: 75-12-7
• Molecular Weight: 45.04
• Beilstein: 505995
• EC Number: 200-842-0
• MDL number: MFCD00007941
• UNSPSC Code: 12352111
• PubChem Substance ID: 329757333

Properties

• grade: ACS reagent; puriss. p.a.
• vapor density: 1.55 (vs air)
• vapor pressure: 0.08 mmHg ( 20 °C); 30 mmHg ( 129 °C)
• Assay: ≥99.5% (GC/T)
• form: liquid
• autoignition temp.: 932 °F
• expl. lim.: 2.7-19 %
• technique(s): PCR: suitable
• impurities: ≤0.02% free acid (as HCOOH); ≤0.1% water
• refractive index: n20/D 1.447 (lit.); n20/D 1.447
• pH: 4-10 (20 °C, 200 g/L)
• bp: 210 °C (lit.)
• mp: 2-3 °C (lit.)
• transition temp: transition temp 2.0-3.0 °C
• density: 1.132 g/mL at 20 °C; 1.134 g/mL at 25 °C (lit.)
• cation traces: Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; K: ≤20 mg/kg; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Na: ≤20 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Zn: ≤1 mg/kg
• suitability: in accordance for miscibility
• SMILES string: NC=O
• InChI: 1S/CH3NO/c2-1-3/h1H,(H2,2,3)
• InChI key: ZHNUHDYFZUAESO-UHFFFAOYSA-N

Description

General description

Formamide is a colorless organic solvent. It has been reported to increase the precision of PCR (Polymerase Chain Reaction) studies. Microwave spectral study of its isotopic forms suggests that the molecule of formamide is nonplanar in which the H₂N-C group exhibits a shallow pyramid like structure.

Application

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H351,H360D,H373
• Precautionary Statements: P201 - P202 - P260 - P280 - P308 + P313 - P405
• Hazard Classifications: Carc. 2 - Repr. 1B - STOT RE 2 Oral
• Target Organs: Blood
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 1
• Flash Point(F): 305.6 °F
• Flash Point(C): 152 °C