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270733

Sigma-Aldrich

Water

suitable for HPLC

Synonym(s):

Aqua, Dihydrogen oxide

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About This Item

Linear Formula:
H2O
CAS Number:
7732-18-5
Molecular Weight:
18.02
Beilstein:
2050024
EC Number:
231-791-2
MDL number:
MFCD00011332
UNSPSC Code:
12190000
PubChem Substance ID:
329752968
NACRES:
NA.21

vapor density

<1 (vs air)

Quality Level

200

vapor pressure

3 mmHg

form

liquid

technique(s)

HPLC: suitable

evapn. residue

<0.0003%

refractive index

n20/D 1.34 (lit.)

pH

6.0-8.0 (25 °C)

bp

100 °C (lit.)

mp

0 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

λ

H2O reference

UV absorption

λ: 205 nm Amax: 0.01
λ: 210 nm Amax: 0.01
λ: 250-400 nm Amax: 0.005

SMILES string

O

InChI

1S/H2O/h1H2

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

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General description

Water suitable for HPLC is commonly used as a solvent for isocratic elution in both cation and anion chromatography. Additionally, it is used as a component to prepare standard stock solutions, and for evaporation under a nitrogen stream in a water bath to concentrate the sample before LC-MS-MS analysis.

Application

Water is used as a:
  • Solvent to prepare buffers during the cell lysis process.
  • Component of the mobile phase during the HPLC–UV–diode-array detection for the separation and detection of the analytes.

Preparation Note

Product filtered through a 0.2 μm filter

Other Notes

Important notice
The article number 270733-6X1L will be discontinued. Please order the single bottle 270733-1L which is physically identical with the same exact specifications.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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N-glycosylation of proteins provides a rich source of information on liver disease progression because majority of serum glycoproteins, with the exception of immunoglobulins, are secreted by the liver. In this report, we present results of an optimized workflow for MALDI-TOF
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Phosphorylation of proteins is an important cellular regulatory process. The analysis of protein phosphorylation is challenging due to the high dynamic range and low abundance natures of phosphorylated species. Mass spectrometry (MS) of phosphopeptides obtained from tryptic protein digests is
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