N4638

Neutral Red

powder, BioReagent, suitable for cell culture

• Synonym(s): 3-Amino-7-dimethylamino-2-methylphenazine hydrochloride, Basic Red 5, Toluylene red
• Empirical Formula (Hill Notation): C₁₅H₁₇ClN₄
• CAS Number: 553-24-2
• Molecular Weight: 288.78
• Colour Index Number: 50040
• Beilstein: 3918740
• EC Number: 209-035-8
• MDL number: MFCD00012651
• UNSPSC Code: 12171500
• PubChem Substance ID: 24897707

Properties

• product line: BioReagent
• Quality Level: 200
• form: powder
• composition: Dye content, ≥90%
• technique(s): cell culture | mammalian: suitable; titration: suitable
• color: brown to black
• pH: 6.8-8.0, red to yellow
• mp: 290 °C (dec.) (lit.)
• solubility: H₂O: 10 mg/mL
• application(s): diagnostic assay manufacturing
• storage temp.: 2-8°C
• SMILES string: Cl.CN(C)c1ccc2nc3cc(C)c(N)cc3nc2c1
• InChI: 1S/C15H16N4.ClH/c1-9-6-13-15(8-11(9)16)18-14-7-10(19(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H
• InChI key: PGSADBUBUOPOJS-UHFFFAOYSA-N

Description

General description

Neutral red (C.l. 50040, Cl Basic red 5) is a protonated diaminophenazine dye that has a small planar conjugated system.

Application

Neutral red has been used in several histological staining applications that include:

• as a nuclear stain
• as a counterstain in the Gram procedure, either alone, or as a component of the Twort stain, a fast green-neutral red mixture.
• fluorescent staining of nucleic acids in nervous tissue, of lipids and hydrophobic substances such as suberin and of phenolic compounds in algal cell walls
• detection of viable yeast cells in dandruff samples and of mussel larvae
• Neutral red has also been used as a stain in flow cytometry.

Useful as an indicator for preparing neutral red paper, and as a biological stain.

Preparation Note

Purified

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves