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11-0398

Sigma-Aldrich

Fluorescamine

≥98.0%

Synonym(s):

4-Phenylspiro-[furan-2(3H),1-phthalan]-3,3′-dione

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About This Item

Empirical Formula (Hill Notation):
C17H10O4
CAS Number:
38183-12-9
Molecular Weight:
278.26
Beilstein:
921143
EC Number:
253-814-5
MDL number:
MFCD00005928
UNSPSC Code:
12352300
PubChem Substance ID:
329749297

Assay

≥98.0%

availability

available only in Japan

mp

153-157 °C (lit.)

SMILES string

O=C1OC2(OC=C(C2=O)c3ccccc3)c4ccccc14

InChI

1S/C17H10O4/c18-15-13(11-6-2-1-3-7-11)10-20-17(15)14-9-5-4-8-12(14)16(19)21-17/h1-10H

InChI key

ZFKJVJIDPQDDFY-UHFFFAOYSA-N

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Application

Non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds. Low background due to hydrolysis. Useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes. Effectively blocks newly generated amino termini in protein sequence analyses.

Suitability

for HPLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
U1290
U6267
U0158
U7543
U9843

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Wen-Hsien Tsai et al.
Journal of chromatography. A, 1217(49), 7812-7815 (2010-11-04)
A simple sugaring-out assisted liquid-liquid extraction method combined with high-performance liquid-chromatography with fluorescence detection (HPLC-FL) was developed for the extraction and determination of sulfonamides in honey. Sample preparation consisted of acid hydrolysis to release sugar-bound sulfonamides. After derivatization with fluorescamine
Manjeet Deshmukh et al.
Biomaterials, 31(26), 6675-6684 (2010-06-22)
Two vinyl sulfone functionalized crosslinkers were developed for the purpose of preparing degradable poly(ethylene glycol) (PEG) hydrogels (EMXL and GABA-EMXL hydrogels). A self-elimination degradation mechanism in which an N-terminal residue of a glutamine is converted to pyroglutamic acid with subsequent
Laura Contreras-Ruiz et al.
Cornea, 29(5), 550-558 (2010-03-26)
Hyaluronic acid-chitosan nanoparticles (HA-CS NPs) have the potential to serve as a reliable drug delivery system to topically treat ocular surface disorders. We evaluated the in vivo uptake by ocular structures, the acute tolerance, and possible alterations of tear film
Grégoire Danger et al.
Electrophoresis, 29(19), 4036-4044 (2008-10-30)
The first results of chiral separations with the gradient elution isotachophoresis method are presented. As previously described, citrate is used in the run buffer as the leading ion and borate in the sample buffer as the terminating ion. Modulation of
José Bernal et al.
Journal of chromatography. A, 1216(43), 7275-7280 (2009-06-10)
A novel method for the simultaneous analysis at trace level of sulfonamides (sulfaguanidine, sulfanilamide, sulfacetamide, sulfathiazole, sulfapyridine, sulfachloropyridazine, sulfamerazine, sulfameter, sulfamethazine, sulfadoxine, sulfadiazine, sulfamonomethoxine, sulfadimethoxine) in honey is described. Methanol has been used in the sample treatment step to avoid
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