11332465001

TWEEN^® 20

non-ionic, aqueous solution, 10% (w/v)

• Synonym(s): TWEEN 20, Tween, Tween 20
• UNSPSC Code: 12352200

Properties

• description: Formula: C₅₈H₁₁₄O₂₆, for n = 20
• form: solution
• mol wt: M_r 1228 (for n= 20)
• packaging: pkg of 5 × 10 mL
• manufacturer/tradename: Roche
• technique(s): cell culture | plant: suitable; microbiological culture: suitable
• CMC: ~0.06 mM
• absorption: = 0.025 at 254 nm at 0.05 % (w/v)
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Tween 20, also referred to as poly(oxyethylene)_n-sorbitan-monolaurate, is a non-ionic surfactant. It is mainly used in membrane research.

Application

Tween 20 is used for:

• solubilizing membrane proteins during isolation of membrane-protein complexes.
• a subsequent protein detection by UV light absorption, due to its low UV absorbance.
• ELISA (enzyme-linked immunosorbent assay) and immunoblots.

Quality

Purity: <2 ppm peroxide content (as H₂O₂), <200 μM carbonyls, <100 μS conductivity. Specially purified for membrane research.

Physical form

10% (w/v) aqueous solution under nitrogen, supplied in injection glass bottles.

Preparation Note

Working concentration: >10 mg/mg membrane lipid

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): No data available
• Flash Point(C): No data available