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Roche

DNA Molecular Weight Marker III, DIG-labeled

greener alternative

10 μg/mL, pkg of 500 μL (10 μg/ml), solution

Synonym(s):

DNA marker

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About This Item

UNSPSC Code:
41105335

form

solution

Quality Level

100

packaging

pkg of 500 μL (10 μg/ml)

manufacturer/tradename

Roche

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

concentration

10 μg/mL

color

colorless

solubility

water: miscible

greener alternative category

Aligned,

storage temp.

−20°C

General description

DNA Molecular Weight Marker III, DIG-labeled is a fragment mixture prepared by cleavage of λDNA with EcoR I and Hind III. The size ranges from 0.12 to 21.2kbp. A photodigoxigenin has been introduced at approx. every 200th to 300th base in the DNA fragments.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Application

DNA Molecular Weight Marker III, DIG-labeled, has been used as a size standard in Southern blot analysis for nucleic acid labeling and detection.

Features and Benefits

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Sequence

The mixture contains 13 fragments wit the following base pair lengths: 125, 564, 831, 947, 1375, 1584, 1904, 2027, 3530, 4268, 4973, 5148, and 21226 bp.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.

Other Notes

For life science research only. Not for use in diagnostic procedures.
Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker the smallest fragments will only be visible on overloaded gels.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

No data available

Flash Point(C)

No data available

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Diane M Ramos et al.
BMC developmental biology, 6, 55-55 (2006-11-23)
Precise temporal and spatial regulation of transgene expression is a critical tool to investigate gene function in developing organisms. The most commonly used technique to achieve tight control of transgene expression, however, requires the use of specific DNA enhancers that
Stéphanie Tomé et al.
PLoS currents, 6, doi:10-doi:10 (2014-03-13)
Many human diseases are associated with the abnormal expansion of unstable trinucleotide repeat sequences. The mechanisms of trinucleotide repeat size mutation have not been fully dissected, and their understanding must be grounded on the detailed analysis of repeat size distributions

Articles

Digoxigenin (DIG) Labeling Methods

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

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