03732738001
Roche
Dideoxynucleoside Triphosphate Set
Sequencing Grade, sodium salt
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.21
Recommended Products
grade
Molecular Biology
Quality Level
Assay
≤1.5% (ddNDP, HPLC)
≥98% (ddNTP, HPLC)
form
liquid
packaging
pkg of 4 × 100 μL (4 x 1 μmol; 10 mM, each)
manufacturer/tradename
Roche
color
colorless
solubility
water: miscible
shipped in
dry ice
storage temp.
−20°C (−15°C to −25°C)
General description
The Dideoxynucleoside Triphosphate Set, Sequencing Grade, sodium salt solution, consists of dideoxynucleotides of very high purity (ddNTP HPLC, area% ≥98%; ddNDP HPLC, area% ≤1.5%), specifically manufactured and tested for sequencing reactions.
The set contains 4 individual vials of ddATP, ddCTP, ddGTP, and ddTTP, each at 10 mM as a clear, colorless solution of the sodium salt (pH 8.3).
The set contains 4 individual vials of ddATP, ddCTP, ddGTP, and ddTTP, each at 10 mM as a clear, colorless solution of the sodium salt (pH 8.3).
Application
2′,3′-Dideoxynucleoside triphosphates inhibit the chain elongation of a given primer catalyzed by the DNA polymerase (e.g., Klenow enzyme) and are therefore used for DNA sequencing according to Sanger (Sanger, F. et al. (1977) PNAS USA 74, 5463).
Sequencing is achieved by including in each reaction a dideoxynucleotide that acts as a chain terminator. Four reactions are set up, each containing the same template and primer, and a chain terminator specific for A, C, G, or T. Because only a small amount of the chain terminator is included, incorporation into the new DNA strand is a random event. Each reaction therefore generates a collection of fragments, and every DNA strand will end at the same type of base (A, C, G, or T).
Sequencing is achieved by including in each reaction a dideoxynucleotide that acts as a chain terminator. Four reactions are set up, each containing the same template and primer, and a chain terminator specific for A, C, G, or T. Because only a small amount of the chain terminator is included, incorporation into the new DNA strand is a random event. Each reaction therefore generates a collection of fragments, and every DNA strand will end at the same type of base (A, C, G, or T).
Packaging
The set contains 4 individual vials of ddATP, ddCTP, ddGTP and ddTTP each at 10 mM as a clear, colorless solution of the sodium salt (pH 8.3).
Quality
Each lot of dideoxynucleotide solution is assayed for function in sequencing reaction.
Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.
Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.
Preparation Note
Activator: Mg2+, Co2+ and Mn2+ ions
Working solution: Preparation of Termination Mix
Before preparing the termination mix, dilute an appropriate volume of each dideoxynucleotide in water to a concentration of 10 μM.
Working solution: Preparation of Termination Mix
Before preparing the termination mix, dilute an appropriate volume of each dideoxynucleotide in water to a concentration of 10 μM.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Kit Components Only
Product No.
Description
- ddATP, sodium salt 10 mM
- ddCTP, sodium salt 10 mM
- ddGTP, sodium salt 10 mM
- ddTTP, sodium salt 10 mM
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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