SCT205
BioTracker™ EP-HMRG Green Protease Live Cell Probe
Synonym(s):
EP protease probe, EP-HMRG live cell probe, EP-HMRG probe, protease live cell probe
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About This Item
UNSPSC Code:
41106514
NACRES:
NB.12
manufacturer/tradename
Millipore
Quality Level
technique(s)
cell based assay: suitable
cell culture | mammalian: suitable
flow cytometry: suitable
shipped in
dry ice
storage temp.
−20°C
General description
BioTracker™ EP-HMRG is nearly nonfluorescent at pH 7 or higher. It fluoresces by the reaction with proteases that specifically digest the EP (glutamic acid, proline) peptide sequence. A green fluorescent product is membrane permeable and localizes to the lysosome.To use the probe, warm the vial to room temperature and add 30 μL of DMSO or PBS to one vial to make 1 mM solution.Dilute the stock solution with a neutral buffer or culture medium to 1-2 uM and use the diluted solution immediately.SPECTRAL PROPERTIES:Excitation: 500 nmEmission: 524 nmFor fluorescence microscopy, blue excitation filter cubes for FITC or GFP are suitable.™ EP-HMRG Live Cell Probe at -20°C, desiccated and protected from light. Centrifuge vial briefly to collect contents at bottom of vial before opening.NOTE: Storage of reconstituted probe is not recommended. For best results, discard unused probe solution and make fresh for each use.
Features and Benefits
Live cell imaging probe that reacts with the proteases that digest the peptide sequence of EP (glutamic acid, proline) to fluoresce green by fluorescence microscopy or flow cytometry.
Target description
Early detection of esophageal squamous cell carcinoma (ESCC) is an important prognosticator, but is difficult to achieve by conventional endoscopy. Since fluorescence-based visualization is considered a promising approach, we aimed to develop an activata
Legal Information
BioTracker is a trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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