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S2EM004M99

Millipore

Multiscreen® 96 well Plate, hydrophobic PVDF membrane

pore size 0.45 μm, sterile

Synonym(s):

Hydrophobic 96-Well Plate, PVDF 96-Well Plate

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About This Item

UNSPSC Code:
41104923
eCl@ss:
32039006
NACRES:
NB.22

material

PVDF membrane (hydrophobic)
acrylic

Quality Level

400

description

Sterile, white 96-well filter plate with 0.45 um pore size Hydrophobic PVDF membrane for ELISPOT Assays. Comes in a pack of 10.

sterility

sterile

manufacturer/tradename

MultiScreen®

parameter

50-250 μL sample volume (per well)

technique(s)

ELISPOT: suitable

filtration area

0.28 cm2

plate size

96 wells

matrix

Immobilon®-P

pore size

0.45 μm pore size

shipped in

ambient

General description

Filter Code: IP

Application

MultiScreen®-IP Filter Plate, 0.45 μm, white, sterile has been used in the enzyme-linked immunospot (ELISPOT) assay.

Physical form

Color Code: White

Other Notes

Automation Compatible: No

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
MULTISCREEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

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Sung-Ho Chang et al.
Cells, 9(5) (2020-05-24)
We investigated the immunogenicity of allogeneic human adipose-derived mesenchymal stem cells (ADSCs) through the production of alloreactive-CD8 T and -memory CD8 T cells, based on their human leukocyte antigen (HLA) expression. In surface antigen analysis, ADSCs do not express co-stimulatory
Daniela Frasca et al.
Vaccine, 34(25), 2834-2840 (2016-04-25)
The success of a vaccine in inducing a protective antibody response depends on the longevity of both long-lived plasma cells (PC) and memory B cells. We have previously shown that the in vivo antibody response to a new influenza vaccine
Sarah Côté-Bigras et al.
Clinical endocrinology, 80(2), 296-300 (2013-06-06)
While thyrotrophin receptor (TSHR) is recognized as the main autoantigen in Graves' disease (GD), the actual antigen specificity of T cells that infiltrate the thyroid and the orbit is unknown. Identifying T cell responses to TSHR peptides has been difficult
Samantha K Greaney et al.
Cancer immunology research, 8(2), 246-254 (2019-12-20)
Whereas systemic IL12 is associated with potentially life-threatening toxicity, intratumoral delivery of IL12 through tavokinogene telseplasmid electroporation (tavo) is safe and can induce tumor regression at distant sites. The mechanism by which these responses are mediated is unknown but is
Kee Thai Yeo et al.
Journal of immunology (Baltimore, Md. : 1950), 202(5), 1465-1478 (2019-01-25)
Infections during pregnancy can expose the fetus to microbial Ags, leading to inflammation that affects B cell development. Prenatal fetal immune priming may have an important role in infant acquisition of pathogen-specific immunity. We examined plasma proinflammatory biomarkers, the proportions

Articles

The ELISpot Assay Enables Functional Analysis of Cellular Immunology

The enzyme-linked immunosorbent spot (ELISpot ) assay enables visualization of multiple secretory products from a single responding cell. The ELISpot provides both qualitative (type of immune protein) and quantitative (number of responding cells) information.

Protocols

IFN-γ ELISpot Assays on MultiScreen® IP

This page shows an example protocol of an ELISpot assay for quantifying the cells producing interferon-gamma in response to antigen or non-specific activation using phytohemagglutinin.

Related Content

Guidelines for Bioassays on MultiScreen Filter Plates: Protein Binding and Cell Loading

Guidelines for various bioassays on the MultiScreen system, which makes it possible to carry out an assay from sample incubation to scintillation counting in a 96-well plate.

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