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PTHT12H48

Millipore

Millicell® 12 well hanging cell culture inserts

pore size 0.4 μm, translucent PET membrane, diam. 12 mm, Tissue Culture (TC)-treated surface, sterile

Synonym(s):

Polyethylene Terephthalate hanging cell culture insert, cell culture inserts, permeable culture insert, plate inserts, tissue culture insert, tissue culture plate

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About This Item

UNSPSC Code:
41122107
eCl@ss:
32011202
NACRES:
NB.13

material

polystyrene housing
translucent PET membrane

Quality Level

200

sterility

γ-irradiated
sterile

feature

100x106 pores/cm2

packaging

pack of 48 ea (individually blister packaged)

manufacturer/tradename

Millicell®

parameter

37 °C max. temp.

technique(s)

cell attachment: suitable
cell differentiation: suitable
permeability assay: suitable

H

15.5 mm

diam.

12 mm

insert maximum volume

0.8 mL

insert working volume

0.4 mL

size

12 wells

surface area

1.1 cm2

color

translucent membrane

pore size

0.4 μm

binding type

Tissue Culture (TC)-treated surface

General description

Millicell® Hanging Cell Culture inserts with Polyethylene Terephthalate (PET) membrane are ideal for the growth and differentiation of various cell types. This individually sterile-packaged insert has a 0.4 μm membrane pore size and fits in most 12-well plastic tissue culture plates.

In plastic tissue culture plates, cells can access media only from their apical sides, but in Millicell® tissue culture inserts, cells can access media from both their apical and basolateral sides. As a result, cell growth, structure, and function more closely mimic what occurs in vivo.

The insert’s flanged design suspends it within the culture plate well and makes it possible to access both sides of the cell monolayer. Spaces between three protruding arms facilitate pipetting between the outside of the insert and the culture plate well.

Application

  • Cell Proliferation
  • Cell Surface Receptors
  • Co-Culture
  • Epithelial Cell Growth
  • Feeder Layers
  • Fluorescent Detection / Immunohistochemistry
  • In Vitro Toxicology
  • Microbial Attachment
  • Polarized Protein Secretions
  • Polarized Uptake
  • Transport / Permeability

Legal Information

Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

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Articles

Barrier Formation and Permeability Assays using Millicell® Hanging Cell Culture Inserts

How to set up barrier formation and permeability assays using cell culture inserts and TEER measurements.

Using Immortalized 16HBE14o- Human Bronchial Epithelial Cell Lines to Model Respiratory Lung Diseases

16HBE14o- human bronchial epithelial cells used to model respiratory epithelium for the research of cystic fibrosis, viral pulmonary pathology (SARS-CoV), asthma, COPD, effects of smoking and air pollution. See over 5k publications.

Protocols

Generating Organotypic Air-Liquid Interface (ALI) 3D Skin Cultures as an In-Vitro Model of the Human Epidermis

3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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